The effect of 6-aminonicotinamide on limb development.
نویسنده
چکیده
The morphological effect of the nicotinamide analogue 6-aminonicotinamide on the development of the embryonic chick limb was studied, with special reference to muscle, cartilage, feather germ formation, and ossification. No evidence was found to support theories of control of differentiation by nicotinamide-like substances, although the effect of nicotinamide analogues has often been quoted as evidence for such theories. However, the effect of 6-AN in shortening the limb provides an opportunity to study the interaction of various tissues in normal growth and development, and the results are used to shed further light on the processes involved in muscle splitting, and feather germ formation.
منابع مشابه
Alterations of chondroitin sulfate synthesized by chick embryo cartilage cultured in the presence of 6-aminonicotinamide.
Treatment of day-4 chick embryos with 6-aminonicotinamide (6-AN) impairs limbs chondrogenesis and produces micromelia. Interference with limb cartilage development may be related to decreased NAD-dependent synthesis of ATP due to the fact that chondrogenesis is dependent upon anaerobic metabolism. To better understand the effect of 6-AN on chondrogenesis, isolated cartilage epiphyses from day-1...
متن کاملImpaired energy metabolism as an initial step in the mechanism for 6-aminonicotinamide-induced limb malformation.
The analogue and antagonist of nicotinamide, 6-aminonicotinamide (6-AN), impairs cartilage formation and results in shortening of the limbs when administered to chick embryos. Studies have shown that 6-AN forms an abnormal NAD analogue which inhibits the activity of NAD-dependent enzymes associated with production of ATP. To determine if an effect on ATP synthesis might be associated with the m...
متن کاملSynergistic antileukemic effect of 6-aminonicotinamide and 1,3-bis(2-chloroethyl)-1-nitrosourea on L1210 cells in vitro and in vivo.
A series of nicotinamide analogs were evaluated for their ability to inhibit L1210 cell poly(adenosine diphosphoribose) polymerase, and also for their ability to potentiate the cytocidal effects of 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU), nitrogen mustard, and gamma-irradiation. In L1210 cells growing in culture and in vivo. In vitro, nicotinamide, 5-methylnicotinamide, 6-aminonicotinamide,...
متن کاملEarly Biochemical Changes in the Embryonic Ra t Heart after Teratogen Treatment
The effects on mucopolysaccharide synthesis in isolated embryonic rat hearts of in vivo and/or in vitro 6-aminonicotinamide, dietary-induced folic acid deficiency, aminopterin, and trypan blue were investigated using uptake of 35S-sodi~m sulfate to measure the rate of mucopolysaccharide synthesis. Intraperitoneal injection of 6-aminonicotinamide on day 11 of gestation caused inhibition of mucop...
متن کاملEarly changes in parathyroid hormone response and proteoglycan synthesis of chick embryonic femur produced by exposure to 6-aminonicotinamide in ovo.
The mechanism of induction of micromelia in 6-day-old chick embryo by 6-aminonicotinamide (6-AN) was investigated. Six-day-old chick embryo exposed to 6-AN did not show micromelia when tenfold excess of nicotinamide over 6-AN was co-administered. The ability of nicotinamide to prevent the induction of micromelia was partially offset after 4 hr of exposure to 6-AN and completely disappeared afte...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Journal of embryology and experimental morphology
دوره 55 شماره
صفحات -
تاریخ انتشار 1980